ogt inhibitor-st045849  (TimTec LLC)

Journal: Life Science Alliance

Article Title: Nutrient sensitive protein O -GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesis

doi: 10.26508/lsa.202201385

Figure Lengend Snippet: (A) Protein expression analysis by Western blotting was performed on embryo brain cortices from E14.5 of control (C), hyperglycemic (HG), and hyperglycemic treated with O -GlcNAc transferase inhibitor, ST045849 (ST) rats using anti- O -GlcNAc, Ezh2-Thr311p, Ezh2-Thr487p, Ezh2, H3K4me2, H3K4me3, H3K27me3, H3, Mll1, Mll2, and Lsd1 antibodies. The expression of β-Actin was used as the loading control. The results are representative of three independent biological replicates. (A, B) Densitometric quantitation of Western blots from panel (A). Data shown are mean ± SD of three biological replicates. Statistical analyses (unpaired t test) were made between C versus HG samples and HG versus ST samples using GraphPad Prism Software. Asterisks represent differences being significant (* P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: For OGT inhibition, pregnant hyperglycemic animals were injected with OGT inhibitor ST045849 (TimTec; 600–1,000 μg/animal) intraperitoneally from E9.5 to E13.5 as per previous report ( ).

Techniques: Expressing, Western Blot, Control, Quantitation Assay, Software

Journal: Life Science Alliance

Article Title: Nutrient sensitive protein O -GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesis

doi: 10.26508/lsa.202201385

Figure Lengend Snippet: (A) Protein expression analysis by Western blotting was performed on embryo brain cortices from E14.5 of control (C), hyperglycemia (HG), and hyperglycemia treated with O -GlcNAc transferase inhibitor ST045849 (ST) rats using anti-Eomes, Tbr1, and β-Tubulin III antibodies. The expression of β-Actin was used as the loading control. The results are representative of two biological replicates. (A, B) Densitometric quantitation of Western blots from panel (A).

Article Snippet: For OGT inhibition, pregnant hyperglycemic animals were injected with OGT inhibitor ST045849 (TimTec; 600–1,000 μg/animal) intraperitoneally from E9.5 to E13.5 as per previous report ( ).

Techniques: Expressing, Western Blot, Control, Quantitation Assay

Journal: Nature Communications

Article Title: O -GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity

doi: 10.1038/s41467-019-13914-8

Figure Lengend Snippet: a 3D-reconstructed confocal images of differentiated C3H/10T1/2 cells treated with DMSO or ST045849 (ST04), an OGT inhibitor, cultured in 0.4 mM oleic acid-supplemented medium and serum-depleted medium, and stained with BODIPY 493/503 (lipid droplets, green), and DAPI (nucleus, blue), scale bar is 20 μm. b , c Cumulative frequency distribution and mean average of lipid droplet size shown in a (three experiments). d Time-lapse imaging of differentiated C3H/10T1/2 cells treated with DMSO and ST04, and labeled with BODIPY 558/568 C12 fatty acid (lipid droplets, red), scale bar is 20 μm.; e Quantification of lipid droplet size shown in d ( n = 3/group, three experiments). Data are presented as mean ± s.e.m. Statistical analysis: ANOVA with Dunnett multiple comparisons for c and Student’s t test for e , ** p < 0.01, and *** p < 0.001. Source data are provided as a Source Data file.

Article Snippet: OGT inhibitor ST045849 (ST04) was purchased from TimTec.

Techniques: Cell Culture, Staining, Imaging, Labeling